Dec 20, 2018 Release
-bnni(reported by Vanessa Vera Fain).
-rcluster-maxactivates relaxed clustering algorithm without having to specify
--no-outfilessuppress .treefile as expected (reported by Cecile Ane).
Oct 28, 2018 Release
Sep 7, 2018 Release
--show-lh(thanks Benjamin Redelings)
-st MULTIoption (requested by Ingo Ebersberger)
--runsand partition model (reported by David Maddison)
Aug 23, 2018 Release
-madd C10(reported by Juergen Strassert).
-bnnioption (reported by Juergen Strassert).
-m ..MERGE..wrong concatenateAlignments with missing data (reported by David Dunchene).
-bsamwith non-partition model (reported by Sarah Jensen).
Jul 1, 2018 Release
This version contains a number of bugfixes and is highly recommended for all users.
-lmapoption (reported by Giap Nguyen).
-bsam) for standard bootstrap (
-b) and jackknife (
-m ...MERGE) (reported by Guoqing Li).
-t RANDOM(reported by Karen Meusemann).
May 8, 2018 Release
Apr 30, 2018 Release
--runsto perform multiple tree searches (issue #64 requested by David Maddison). This is motivated by a recent evaluation (Zhou et al. 2018) showing that 10 independent runs outperformed single run in terms of likelihood maximisation.
--show-lhto compute tree log-likelihood without parameter optimisation (issue #67).
+Pfor PoMo model string (thanks Carolin Kosiol).
GTR20(reported by Dominik Schrempf).
-alrt(reported by Guifre Torruella Cortes).
+ASCmodel when some states are rare (reported by Paul Madeira).
-zoption with rooted trees in the presence of identical sequences (reported by cjp1043).
Mar 22, 2018 Release
This version improves software stability and highly recommended to update for all users.
-t) and partition model (reported by Dieter Waechter).
-spoption (edge-unlinked) and
Too many iterations in tqlito WARNING.
-mtree(reported by Mark Miller).
-sppby temporarily turning on NNI5 instead of NNI1 (originally reported by Xiaofan).
-ntmaxoption to specify maximum number of threads by
-nt AUTOoption (requested by @sjspielman).
-j(requested by Emmanuel Toussaint).
-versionto display version number (requested by David Maddison).
Mar 1, 2018 Release
LG+C10or similar models (reported by Craig Herbold)
-netoption (reported by Cameron Weadick)
--write-branchesto write branch lengths of tree (and partition trees) into
.branches.csvfile (requested by Rob Lanfear)
-ooption to specify a comma-separated list of outgroup taxa (requested by Andrew Roger)
Jan 1, 2018 Publication
MPBoot is an open-source and efficient program to reconstruct maximum parsimony phylogenetic trees for large DNA and protein sequence alignments. Importantly, MPBoot provides a fast approximation for maximum parsimony bootstrap, inspired by a similar methodology for maximum likelihood (Minh et al., 2013). If you use MPBoot in a paper please cite:
D.T. Hoang, L.S. Vinh, T. Flouri, A. Stamatakis, A. von Haeseler, and B.Q. Minh (2018) MPBoot: fast phylogenetic maximum parsimony tree inference and bootstrap approximation. BMC Evol. Biol., 18, 11. https://doi.org/10.1186/s12862-018-1131-3
For feedback or bug reports please post a question to the IQ-TREE forum:
Precomplied executables are provided for computers with SSE4 or AVX support:
tar xvzf mpboot-avx-1.1.0-Linux.tar.gzunder Unix). This should create a directory
binfolder inside the extracted folder. You can rename it to
mpbootand copy to your system search path such that you can invoke it by entering
mpbootfrom the terminal.
In case you cannot find executables for your platforms or want to compile the source code:
buildin the uncompressed source code folder:
cmake .. -DIQTREE_FLAGS=sse4 -DCMAKE_C_COMPILER=clang -DCMAKE_CXX_COMPILER=clang++
avxin above command if you decide to run MPBoot on AVX-supported machines.
mpboot-avx) once the make command is done.
mpboot with actual path to executable)
Reconstruct maximum parsimony tree from a sequence alignment (
mpboot -s example.phy
Reconstruct MP tree and assess branch supports with the MPBoot method (1000 replicates):
mpboot -s example.phy -bb 1000
Display all usage options:
Input alignment file
example.phy is in PHYLIP format. MPBoot also supports FASTA or NEXUS format.
|Option||Usage and meaning|
|-mulhits||Store multiple equally parsimonious trees per bootstrap replicate|
|-ratchet_iter ||Number of non-ratchet iterations before each ratchet iteration (default: 1)|
|-ratchet_wgt ||Weight to add to each site selected for perturbation during ratchet (default: 1)|
|-ratchet_percent ||Percentage of informative sites selected for perturbation during ratchet (default: 50)|
|-ratchet_off||Turn of ratchet, i.e. Only use tree perturbation|
|-spr_rad ||Maximum radius of SPR (default: 6)|
|-cand_cutoff <#s>||Use top #s percentile as cutoff for selecting bootstrap candidates (default: 10)|
|-opt_btree_off||Turn off refinement step on the final bootstrap tree set|
|-nni_pars||Hill-climb by NNI instead of SPR|
|-cost ||Read |
The code was originally derived from the IQ-TREE software and the Phylogenetic likelihood library.
D.T. Hoang and L.S. Vinh were financially supported by Vietnam National Foundation for Science and Technology Development (Grant #102.01-2013.04). B.Q. Minh and A. von Haeseler were supported by the Austrian Science Fund (FWF I-2805-B29), T. Flouri and A. Stamatakis by the German Science Foundation (DFG STA860-6/1), and the Klaus Tschira Foundation.